: The 6546 model uses 10 GHz as the only calibration mode.

: Set the flow rate to 0.4 or 0.5 mL/minute and perform 5 injections to clean the injector path. 3. Tuning and Calibration

: Use LCMS-grade deionized water with 0.1% formic acid (Channel A) and 100% LCMS-grade acetonitrile (Channel B).

: For fragile molecules, the system uses lower voltages. If you select the "Fragile Ions" setting, never select High Sensitivity as the Slicer Mode.

: Use the Agilent Site Preparation Checklist to verify all supplies and utilities are ready. 2. System Checkout and Conditioning

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