Aspirate and dispense your peptide sample through the ZipTip.
In scientific research, are a common tool used for purifying and concentrating peptide samples before analysis. Because "C18Zip" is a laboratory product rather than a document title, "generating a full text" typically refers to the Standard Operating Procedure (SOP) for using them. C18zip
Repeat . This prepares the resin to bind the peptides. 4. Sample Binding Aspirate and dispense your peptide sample through the ZipTip
50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste. Repeat
Peptides dissolved in 0.1% Trifluoroacetic acid (TFA). Wetting Solution: 100% Acetonitrile (ACN). Equilibration/Wash Solution: 0.1% TFA in water.
💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry.
Dispense the liquid into a clean, sterile microcentrifuge tube.