: Guidance on Reverse Transcriptase for cDNA synthesis, Alkaline Phosphatase for dephosphorylation, and Terminal Deoxyribonucleotidyl Transferase.
: Detailed properties and nomenclature for DNases (I and II) and specialized enzymes like BAL 31 and Mung-bean nuclease.
: Focus on DNA polymerases, Taq polymerase for PCR, and eukaryotic nuclear RNA polymerases. Methods in Molecular Biology • 16 Enzymes of Mo...
: While published in 1993, the volume captures the era when molecular cloning became a routine laboratory technique, providing the rigorous biochemical groundwork still relevant for understanding modern variants of these enzymes.
The book , edited by Michael M. Burrell , is the 16th volume in the renowned Methods in Molecular Biology series. Published in May 1993 by Humana Press, it serves as a foundational manual for researchers needing detailed information on the properties, nomenclature, and practical application of enzymes used as tools in molecular cloning and analysis. Core Content and Themes : Guidance on Reverse Transcriptase for cDNA synthesis,
Nucleases, Polymerases, Restriction Enzymes, Ligases, Proteases Enzymes of Molecular Biology (Methods in ... - Amazon.com
: Each chapter typically includes an introduction, list of necessary materials/reagents, and readily reproducible protocols with troubleshooting tips. Quick Reference Table Editor Michael M. Burrell Series Editor John M. Walker Publisher Humana Press (now part of Springer Nature) ISBN 978-0-89603-234-7 Major Topics : While published in 1993, the volume captures
The volume is structured into (approximately 380 pages) that provide a mix of theoretical background and step-by-step laboratory protocols. It addresses a critical gap: while protocols are widely available, detailed information on the specific nature and reaction requirements of the enzymes themselves is often scattered in older literature. Key enzymatic groups covered include: